Protein_Domain

Part:BBa_K351007:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-10-25)


first Ig-like region of Human basic FGFR with signal peptide for s.pombe surface


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
    Illegal NotI site found at 487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With this design, the signal peptide should be changed when the expression organism is changed.


Source

This sequence comes from the hybrid of two sequence. The nucleotide sequence for signal peptide is from S.pombe,MVFLNSSPFKGRLLFFVYLLIISTRLVAA of Cell wall integrity and stress response component 1 protein. and ig-like region is from human basic FGFR.

References